Qualitative Analysis on the Phytochemical Compounds and Total Phenolic Content of Cissus hastata (Semperai) Leaf Extract

Plant extracts of Cissus hastata, indigenously known as Semperai, have been used an effective traditional remedy against coughs. Recently, the leaf extract was potentially shown to anti-hemorrhoid activity, although there is a lack scientific data due its folklore usage. Hence, therapeutic properties phytochemicals and metabolites Semperai remain elusive. Therefore, this study aims determine total phenolic content phytochemical compounds plant extract. Total antioxidant activity were determined by Folin–Ciocalteau method 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, respectively. Phytochemical present in methanol analyzed qualitative method. Results showed comprised 21.3 mg GAE/g with reference gallic acid. The almost absence IC50 7.80 µg/mL when compared trolox Presence red orange precipitate acid indicate alkaloid content, while appearance black-blue/green color are referred tannins. steroids represented upper layer yellowish sulfuric green fluorescence comparison cholesterol. Nonetheless, saponin not detected extract, indicated persisting foam test solution sodium dodecyl sulphate. In conclusion, despite having property, fair amount compounds, including tannins, alkaloids, steroids, which, potentially, highly anti-inflammatory towards hemorrhoids.